Pdf enzymatic activity of fungi endophytic on five medicinal. Endophytic fungi from frankincense tree improves host. In these ecosystems, the vegetation is dominated by a particular group of plant species that provide a unique environment harboring diverse groups of microorganisms, including. The lowest extracellular enzyme activity was observed by isolates no 1. The ltdbat enzyme was heterologously expressed and purified. Screening endophytic fungi for production of extracellular enzyme. Evaluation of antifungal and enzymatic potential of. Currently, marine microorganisms are attracting increasing attention as a resource for new enzymes because the enzymes derived from marine microbes are relatively more stable and active than the corresponding enzymes derived from plants or animals lam 2006. Cellulase production from species of fungi and bacteria. Endophytic fungi exhibit a complex web of interactions with host plants and have been extensively studied over the last several years as prolific sources of new bioactive natural products. Production of amylase enzyme in fungi is known to depend on both morphological and metabolic state of the culture.
Endophytic fungi do not always cause plant disease, and they include fungi that yield different effects, including mutual bene. Laccase activity was tested by growing the fungi on gyp agar medium amended with 1naphthol 0. Pdf on sep 15, 2019, hala farouk and others published hydrolytic enzyme production of endophytic fungi isolated from soybean glycine max find, read and cite all the research you need on. Isolation and screening of extracellular enzymatic. Extracellular enzymatic activities of endophytic fungi. Other three strains showed high production of extracellular protease, isolates no 4, 5, and 6 are 155,175 and 149 uml respectively. The pca model was built with three different extracellular enzymesglucosidases, phosphatases and cellulasesand a full crossvalidation was used to validate the enzyme.
Evaluation of fungal endophytes for lignocellulolytic. Endophytic fungi, mostly belonging to the ascomycota, are found in the intercellular spaces of the aerial plant parts, particularly in leaf sheaths, sometimes even within the bark and root system without inducing any visual symptoms of their presence. Screening, isolation, biochemical and plant growth promoting characterization of endophytic bacteria. The compounds ethyl butyrate, ethyl acetate and methyl propionate were used as standards esters which were hydrolyzed by extracellular enzyme from endophytic fungi ec. Nagarajan a, thirunavukkarasu n, suryanarayanan ts, gummadi sn. Extracellular enzymatic activity of endophytic fungal strains. Endophytic fungi were isolated from leaf with midrib, without midrib, petiole, stem. Production of anticancer enzyme asparaginase from endophytic. The aim of the present study is to evaluate woodinhabiting fungal endophytes for lignocellulolytic enzyme production and wood biodegradation. Isolation and screening of extracellular enzymatic activity. The enzyme laccase production by the endophytic fungi will result in the oxidation of colorless 1naphthol to a brownishviolet. The enzyme production and regulation by the endophytic fungi is due to its genetic recombination with the host which has evolved with evolutionary time priest, 1984. Rhizophora annamalaiana, rhizophora appiculata, suaedo monoica, ceriops decandra and rhizophora mucarneta. Chitinase production by endophytic streptomyces aureofaciens cmuac and its antagonism against phytopathogenic fungi.
This study was conducted to isolate lasparaginaseproducing endophytic fungi from medicinal plants of family asteraceae. Asparaginase is an important anticancer enzyme, endophytic fungi eurotium sp. These endophytic fungi were characterized morphotypically using lactophenole cotton blue using scotch tape techniques table 1. Screening, isolation, biochemical and plant growth.
Hydrolytic enzyme production of endophytic fungi isolated from. Enzyme from endophytic fungi and its application for glucose production by yeiti marlida may 2001 chairman. Xylanolytic enzymes were produced in both media, and the total xylanase. The endophytic fungi that have been reported to be xylanase producers include alternaria alternata, hymenoscyphus ericae, and aspergillus terreus. A total of eight endophytic fungi were isolated from leaves of c. This study provides an effective approach for improving taxol production of endophytic fungi by the genetic engineering of taxol biosynthetic pathway genes in the future. Ligninolytic enzymes from tropical endophytic xylariaceae. This study showed that endophytic fungi from asteraceae members have a high lasparaginaseproducing potential and they can be used as an alternative source for production of anticancer enzymes.
Enzymes introduction endophytic fungi exhibit complex interactions with their hosts. Growth of mycelium is crucial for extracellular enzymes 8. However, the enzyme mixtures produced in such media are poorly defined. Isolated endophytic fungi were screened qualitatively and quantitatively for production of extracellular enzymes such as amylase, protease, cellulose and lipase. Molecular identification, extracellular enzyme production.
In the present study screening of endophytic fungi for production of enzyme from medicinal plant azadirachta indica a. Production of endoglucanases is widely distributed among fungi and cellobiohydrolases have been isolated in multiple whiterot fungi and in plant pathogens. Screening of endophytic fungi isolated from azadirachta. Isolation and identification of lasparaginaseproducing. Isolation and enzyme bioprospection of endophytic bacteria. On the basis of the results obtained, the role of endophytic fungi in the wood biodegradation process and their potential applications are discussed. Lasparaginase is an important anticancer enzyme that is used in the first line treatment of acute lymphoblastic leukemia. Diversity and extracellular enzymes of endophytic fungi. Each isolate was able to produce one or the other extracellular enzymes table 1. Roscoe article pdf available in brazilian journal of microbiology 433. Endophytic fungi generally reside asymptomatically in the tissues of higher plants and generally are source of original amidase group of enzymes. Jul 28, 2014 the mangrove ecosystem is a coastal tropical biome located in the transition zone between land and sea that is characterized by periodic flooding, which confers unique and specific environmental conditions on this biome.
Endophytic fungi are microorganisms that colonize plant tissues intercellularly and. Molecular identification, extracellular enzyme production and. Active antiacetylcholinesterase component of secondary. Seven healthy medicinal plants from family asteraceae were selected for the isolation of endophytic fungi using standard surface sterilization techniques.
Enzymes from microbial sources are more stable, economical and are. An endophytic fungus lives within a healthy plant during certain stages of, or throughout, its life cycle. Enzymatic potential and biosurfactant production by. Endophytic microbes, which can be bacteria, fungi or yeast, live symptomless in various part of the host plant. Amylase, cellulase, endophytic fungi, lipase, protease, western ghats, xylanase. Abid ullah 1, hafsa mushtaq 2, usman ali 1, hakim 1, ehsan ali 3, samavia mubeen2 1department of plant science and technology, huazhong agricultural university, wuhan, hubei, p. Isolation and screening of extracellular proteases. Asparaginase production by endophytic fungi from thai. Extracellular enzymatic activities of endophytic fungi isolated from. The selected endophytic fungi aspergillus niger dr02, trichoderma atroviride dr17.
Pdf chitinase production by endophytic streptomyces. Introduction produce extracellular hydrolases as a resistance. Hence, can conclude that endophytic fungi eurotium sp. Extracellular tannase production by endophytic hyalopus sp.
Pdf on sep 15, 2019, hala farouk and others published hydrolytic enzyme production of endophytic fungi isolated from soybean glycine. Isolation, morphological characterization and molecular. This study provides an effective approach for improving taxol production of endophytic fungi by the genetic engineering of. The lowest extracellular enzyme activity was observed by isolates no 1 and 3 with enzyme activity 55 and 75uml respectively. In general, microorganisms have abilities to degrade lignocellulosic compounds. Endophytic fungi extracellular enzymes medicinal plants. Three discs were placed on petri plate containing autoclaved 15 ml of glucose yeast extract peptone agar gyp medium glucose 1 g, yeast extract 0.
This paper focuses on ligninolytic enzyme production by 581 endophytic xylariaceae strains isolated from healthy tropical native plants of northern thailand. Pdf hydrolytic enzyme production of endophytic fungi. In the present study, 32 strains of endophytic fungi were isolated from mangrove plants viz. Asparaginase production by endophytic fungi regular. Various physical and chemical factors have been known to affect the production of amylase such as temperature, ph, carbon sources acting as inducers, and nitrogen. All isolates were subcultured routinely and maintained in the department. Asparaginase production by endophytic fungi from thai medicinal plants. Ac7 showed highest amylolytic activity and was taken for further study. Isolation and screening of extracellular proteases produced. Aug, 2014 endophytic fungi, mostly belonging to the ascomycota, are found in the intercellular spaces of the aerial plant parts, particularly in leaf sheaths, sometimes even within the bark and root system without inducing any visual symptoms of their presence. For this reason, fungi strains isolated from unusual environments have been sought as alternative sources of hydrolytic enzymes 5,6.
Extracellular enzymatic activity of endophytic fungal. There are no reports of amylase production from endophytic fungi isolated. The selected endophytic fungi aspergillus niger dr02, trichoderma atroviride dr17 and dr19, alternaria sp. Stability of enzyme at different temperature and ph range making it suitable for wide array of pharmaceutical applications. The capability of endophytic fungi for production of. Boswellia sacra, an economically important frankincenseproducing tree found in the desert woodlands of oman, is least known for its endophytic fungal diversity and the potential of these fungi to produce extracellular enzymes and auxins. They produce secondary metabolites that generated oligosaccharide degrading enzymes, growth.
Screening of endophytic fungi for amylase production on solid media endophytic fungal culture grown on pda was cut into 5 mm discs with the help of borer. Endophytic fungi are potentially amongst the most interesting microorganisms for screening for the production of industrial biocompounds. Tannase production by endophytic fungi has still remained unexplored. These fungi appear to have a capacity to produce a wide range of enzymes and secondary metabolites exhibiting a variety of biological activities. Cellulose, pectinase, chitinase and lignin using cda media which added czapek dox agar cda. Influence of various physical and chemical factors such as ph, temperature, carbon and nitrogen sources on amylase production in liquid media were studied.
However, the enzymes produced by endophytic fungi have been barely exploited shubha and srinivas 2249. Pdf fungal species that establish an endophytic role inside the tissues of medicinal plants are known to produce a wide range of biologically active. Out of the 19 endophytic isolates only 12, endophytic fungi produced cellulase enzyme and this enzyme involved in biofuels production. In order to identify fungi that produced enzyme with. Oacetyltransferase dbat is a key ratelimiting enzyme of the taxol biosynthetic pathway, which is uncharacterized in taxolproducing endophytic fungi. Among the biotechnological potential of the microbiota from mangroves, it is noteworthy about endophytic fungi, which can be considered as effective sources of different bioactive compounds. Jun 01, 2012 production of amylase enzyme in fungi is known to depend on both morphological and metabolic state of the culture. Production of bioactive secondary metabolites from. Evaluation of fungal endophytes for lignocellulolytic enzyme. We isolated various fungal endophytes belonging to eurotiales 11. Regarding the endophytic fungis potential for extracellular enzyme production, fluorogenic 4methylumbelliferone standards and substrates were used to determine the presence of cellulases, phosphatases and glucosidases in the pure culture. Dr45, annulohypoxylon stigyum dr47 and talaromyces wortmannii dr49 are excellent producers of hydrolytic enzymes to be used as part of blends to decompose sugarcane biomass at industrial level. The characterization of isolates with distinct enzyme production. China 2department of plant sciences, faculty of biological sciences, quaidiazam university, islamabad, pakistan.
Transformation of taxolproducing endophytic fungi by. Mobility of band correspond protein marker of molecular weight 14,300 kd approximately figure 8. Extracellular enzymatic profiles and taxonomic identification. Fungal enzymes are one of them which are used in food, beverages, confectionaries, textiles and leather industries to simplify the processing of raw materials. As compared to the shmm, cultivation of the fungus in lbsm significantly elevated the fpase and cmcase activity table 2. The tips of fungal mycelia were punched aseptically using a sterile cork borer to a diameter of 5 mm and the mycelial disks were placed on specific solid medium for the analysis of the. This current approach aimed to isolate endophytic fungi from the roots, stems and leaves of soybean glycine max l. Aug 23, 2011 spent hydrolysates from bioethanolic fermentation processes based on agricultural residues have potential as an abundant and inexpensive source of pentose sugars and acids that could serve as nutrients for industrial enzyme producing microorganisms, especially filamentous fungi. Amylase production by endophytic fungi cylindrocephalumsp. They are widely used for the production of antibiotics, vitamins, anticancer and. Pdf hydrolytic enzyme production of endophytic fungi isolated. Fungal tannase production has been investigated through liquid surface, submerged and solid state fermentations banerjee et al.